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OTOFPhase 1/2Actively Recruiting

AK-OTOF-101

Study sponsor: Akouos, Inc. / Eli Lilly

A dose-escalation and bilateral-expansion study of a dual-AAVAnc80 OTOF construct delivered with a dedicated intracochlear administration system.

This is an investigational therapy. Inclusion here does not mean it is approved, available, safe, or effective.

Plain-language summary

Study overview

Sources
Current study status
Actively Recruiting
Who is being studied
Cohort 1a: 7–17 years Cohort 1b: first 3 participants are 2 years and older, next 3 may be any age Cohort 2: first 3 participants are 2 years and older, next 3 may be any age bilateral expansion: any age
How treatment is given
One-time intracochlear administration using the Akouos delivery device
Study start date
Sep 15, 2023

Study start date is reported by ClinicalTrials.gov and may differ from the date the first participant received treatment.

Official study record

NCT05821959 · Status shown: Actively Recruiting · Registry information last checked Mar 4, 2026

Terminology Dictionary
Target gene
The gene the treatment is designed to address.
Gene replacement
Delivery of a working gene copy intended to restore a missing or impaired function.
Vector / payload
The delivery vehicle and the genetic material it carries.
Promoter
A DNA control sequence that helps determine where and how strongly the delivered gene is active.
Cochlear access
The route used to reach the inner ear and administer the treatment.
Laterality
Whether treatment is given to one ear or both ears.
Phase 1 / 2
An early human study designed mainly to examine safety, dosing, and initial signs of biological or clinical effect.
Early clinical
An early human study for which a formal clinical phase is not assigned or publicly stated.
Biallelic
A disease-causing change is present in both copies of a gene.
Dual vector
Two delivery vehicles carry separate parts of a gene that is too large to fit into one vehicle.
Intracochlear
Delivered into the fluid-filled inner-ear structure involved in hearing.

Who is being studied

The population described by the public study record.

Study population
People with OTOF-mediated sensorineural hearing loss
Age range
Cohort 1a: 7–17 years Cohort 1b: first 3 participants are 2 years and older, next 3 may be any age Cohort 2: first 3 participants are 2 years and older, next 3 may be any age bilateral expansion: any age
Planned enrollment
22
Study regions
Canada, Taiwan, United Kingdom, and United States

This summary describes the study population; it cannot determine whether an individual qualifies.

Therapy design

The genetic treatment and how its activity is controlled.

Treatment approach
Gene replacement
Delivery vector and gene cargo
Dual AAVAnc80 vectors carrying 5′ and 3′ components of approximately 6 kb human OTOF cDNA
Promoter
Ubiquitous promoter; specific identity is not publicly disclosed

Delivery to the inner ear

How the therapy is administered and whether one or both ears are treated.

How it is delivered
One-time intracochlear administration using the Akouos delivery device
How the inner ear is reached
Transcanal tympanotomy to the round-window membrane with a stapes-footplate vent
Fenestration
Yes — stapes footplate vent (fenestration)
One or both ears
Unilateral cohorts; bilateral expansion
Study start date
Sep 15, 2023

Public evidence reviewed

Evidence and limitations

Current through Jul 14, 2026

The registry anchors the clinical record. Akouos technical material resolves the two-component vector, payload scale, promoter class, round-window route, pressure vent, and intended human corridor; the exact promoter identity remains undisclosed.

Details not publicly disclosed

  • Specific promoter identity
Open the field-by-field evidence map →
Educational resource. This page summarizes public evidence and cannot determine whether someone qualifies for a study. Recruitment status, eligibility, and locations can change. Confirm current details with ClinicalTrials.gov and the study team, and discuss medical decisions with a qualified clinician.